Dr.Yuan Gu
Capital Medical University, China
Title: Vaccination with a multi-epitope protein vaccine encoding T and B-cell epitopes of Trichinella spiralis paramyosin elicits partial protection in mice
Biography
Biography: Dr.Yuan Gu
Abstract
Trichinellosis is a world widespread zoonosis caused by infection of Trichinella spiralis (T. spiralis) through ingesting contaminated pork or other meat. Interrupting parasite transmission via vaccination of livestock with potent vaccine is a practical approach to prevent trichinellosis in China. Paramyosin of T. spiralis (Ts-Pmy) has been proved to be a good vaccine candidate against trichinellosis according to our previous work. As humoral immunity plays an important role in the elimination of the parasite, a synthetic gene containing a protective B-cell epitope and four Th2 epitopes of Ts-Pmy identified before was cloned into the eukaryotic expression vector pET-28a and the recombinant multi-epitope protein (rMEP) was purified. Mice immunized with rMEP formulated with ISA50v2 adjuvant exhibited a significant reduction in muscle larvae burden (55.4%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rMEP-induced protection was associated with a high level of specific anti-rMEP IgG antibodies and a Th1/Th2 mixed immune response. This research laid a foundation for designing effective epitope-based multivalent subunit vaccine against trichinellosis.